Egg White Lysozyme

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Precursor of Egg White Lysozyme

Lysozyme mRNA was translated in a reticulocyte lysate with mixtures of radioactive amino acids. The in vitro product isolated by immunoprecipitation was shown by gel electrophoresis, peptide mapping, and sequence analysis to be larger than lysozyme synthesized in uiuo. An NH,-terminal extension was completely sequenced by automated Edman degradation; the phenylthiohydantoins from each cycle wer...

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Hen Egg - white Lysozyme Crystals

Proton tautomerism is a general phenomenon in organic molecules and plays a vital role in many fields of chemistry and biochemistry. The tautomerism of salicylideneanilines [eq(1)] has attracted a considerable attention because it is closely related to thermoand photochromism. Salicylideneanilines greatly favor the enol form over the cis-keto form in the gas phase. We demonstrate here that the ...

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Characterization of the unfolding pathway of hen egg white lysozyme.

After the recent discovery of a ribonuclease A unfolding intermediate [Kiefhaber, T., et al. (1995) Nature 375, 513-515], we investigated the unfolding pathway of hen egg white lysozyme. At pH* 4.00 with D2O at 10 degrees C and 6 M guanidinium chloride, unfolding shows a single, slow kinetic phase, with a relaxation time of 3300 s when monitored by circular dichroism (CD). Exchange of the trypt...

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Amino acid sequence studies on bobwhite quail egg white lysozyme.

To test the immunological prediction that there should be two amino acid sequence differences between the lysozymes of the bobwhite quail and the chicken, the sequences of these two lysozymes have now been compared. Lysozyme purified from bobwhite quail egg white was reduced, carboxymethylated, and digested with trypsin. The resulting 18 peptides were separated and their compositions determined...

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Mineralization of CaCO3 in the presence of egg white lysozyme.

The influence of egg white lysozyme on the size, shape, crystallography, and chemical composition of amorphous calcium carbonate (ACC) particles obtained from aqueous CaCl2-dimethyl carbonate (DMC)-NaOH solutions was studied. At the onset of precipitation, the presence of lysozyme led to much smaller particles (50-400 nm spherical amorphous lysozyme-calcium carbonate particles (Ly-ACC)) than th...

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ژورنال

عنوان ژورنال: Poultry Science

سال: 1955

ISSN: 0032-5791

DOI: 10.3382/ps.0340679